Project Details
Project Overview
Calibrating a spectrophotometer ensures accurate measurements by aligning it with known reference standards. The process involves several steps:
Wavelength Calibration: Ensures the spectrophotometer’s accuracy at specific wavelengths. This is typically done using standard solutions with known absorbance peaks.
Stray Light Correction: Accounts for any light that reaches the detector without passing through the sample. This is critical for accurate measurements, especially at lower absorbance levels.
Baseline Correction: Adjusts for any background signal present in the absence of a sample. This ensures that only the sample’s absorbance is measured.
Sensitivity Calibration: Determines the spectrophotometer’s sensitivity to absorbance changes. This may involve using standard solutions with known concentrations to verify the instrument’s response.
Linearity Check: Confirms that the instrument’s response is linear across the range of interest. Standard solutions with varying concentrations are typically used to assess linearity.
Validation: Finally, the calibration process is validated by comparing measurements against certified reference materials or established methods to ensure accuracy and reliability.
Regular calibration and maintenance are essential to ensure the continued accuracy and reliability of spectrophotometric measurements.